ROOIBOS ANALYSIS

Research

ROOIBOS ANALYSIS – SUMMARY OVERVIEW

 JULY 2009

 THE ANALYSIS - PROCEDURES

  • Samples of Rooibos were obtained from processors (according to specifications) and submitted to a range of analytical services suppliers for analysis during April 2009.
  • All laboratories were SANAS-accredited, except the Central Analytical Facility at Stellenbosch University – a highly specialized laboratory.
  • The analysis was done on an extract, prepared according to specified standard method.
  • Moisture and ash determinations, as well as microbiological analysis, were also done on dry leaves.

 

THE RESULTS

  • All results are summarized on next page in TABLE 1A.
  • Protein, Fat, Energy: Rooibos (the extract, not the leaves) contains NO fat, protein or kilojoules.
  • Heavy metals: Results low or mostly absent (need to verify permissible levels for Rooibos).
  • Minerals and trace elements: All values were found to be too low to make health claims – see TABLE 2B.
  • Vitamin A and Cwere below detection limit – no claims can be made at all.
  • Some of the results were in line with current values printed on some Rooibos packaging, but others need to be revised – see TABLE 2A.
  • Individual test results have been sent to suppliers.
  • NB:Gerda de Wet (Rooibos Ltd) suggested that all suppliers should use average values, because it may lead to confusion if every supplier uses their specific results.
  • Caffeine- Tests confirmed that Rooibos contains NO caffeine.
  • Microbiological quality: Most of the samples were of very high microbiological quality.
    There were no Salmonella or E coli in any of the samples. Only six of the samples had more than 75 000 CFU/g – therefore exceeded the maximum limit for TMA (Total Microbial Activity) export standards according to current information on the DoA web site (DoA export standards for Rooibos is maximum 150 000 CFU/g for retail packages and 75 000 CFU/g when packed in bulk containers.) It is possible that these samples were not pasteurized (although we asked processors to provide pasteurized samples).

 

Future tests

  • Analysis for polyphenols not included at this time; Prof Lizette Joubert is busy with an extensive study on this.
  • Tannins – need to find an appropriate method applicable to Rooibos. Prof Joubert is investigating. (Already found that HPLC is not an option and methods suitable for wine and fruit juices will not necessarily be applicable to Rooibos.)

 

NUTRITIONAL TABLE FOR ROOIBOS TEA- According to SA Label Legislation :: 

  • The table below provides the typical nutritional information for rooibos tea (traditional and gree rooibos) to be printed on all Rooibos tea pacaking in future. It is in line with the latest food labelling legislation published on 1 March 2010 - Regulation 146 of the Foodstuff, Cosmetics and Disinfectants Act (Act 54 of 1972) that comes in to effect on 1 March 2011

ROOIBOS TEA: TYPICAL NUTRITIONAL INFORMATION*

 

 

Per 100 ml tea

Per single serving (200 ml tea)

Energy (kJ)

0

0

Protein (g)

0

0

Glycaemic carbohydrate (g)
of which total sugar (g)

0
0

0
0

Total fat (g)
of which saturated fat (g)

0
0

0
0

Dietary fibre (g)**

0

0

Total sodium (mg)

3.8

7.6 

 

 TABLE 1A: AVERAGES OF RESULTS OBTAINED ON 25 SAMPLES ANALYSED

 

Type of analysis

Content

Lowest

Highest

Average

Nutritional content

3 blended samples analysed
at CSIR

Moisture
(dry leaves)

7.8 g/100g

9.5 g/100g

8.4 g/100g
(Legal limit: 10%)*

Ash - dry leaves

2.4 g/100g

3.0 g/100g

2.8 g/100g

Ash

<0.2 g/100g

Fat

<0.2 g/100g

Kilojoules

Not detected

Protein

Not detected

Vitamin A

<1667 IU

Vitamin C

<5 mg/1000g

Dietary Fibre

0.2 g/100g

0.4 g/100g

0.3 g/100g

Mineral / trace element analysis at BEMLAB – 25 individual samples analysed
(results in mg/litre)

Calcium (Ca)

2.3

12.3

5.32

Iron (Fe)

0.04

0.15

0.06

Copper (Cu)

0.01

0.03

0.02

Magnesium (Mg)

4.61

12.69

8.00

Manganese (Mn)

0.12

0.27

0.16

Potassium (K)

27.70

52.07

38.66

Sodium (Na)

27.72

54.26

38.02

Zinc (Zn)

0.02

0.06

0.04

Selenium (Se)

0

0.008

0.004

Fluoride tested at CSIR
(9 blended samples)

Fluoride

<1.0 mg/1000g

Microbiological quality (dry leaves) 25 individual samples tested at SWIFT Micro Labs

Total microbial activity

2 300 CFU/g
(CFU = colony forming units per gram)

460 000 CFU/g

47 100

Legal limit: 300 000 CFU/g for retail and 150 000 CFU/g for bulk (According to DoA web site)

Escherichia coli

No E coli detected in any sample

Legal limit: 20 CFU per gram

Salmonella

No salmonella detected in any sample
Legal limit: 0

Caffeine – tested at Central Analytical Facility,

StellenboschUniversity
(3 blended samples)

No caffeine found

 

TABLE 1B - RESULTS FOR HEAVY METAL CONTENT

 

Mineral content analysis at BEMLAB – 25 individual samples analysed
(all results given in mg/litre)

Aluminium (Al)

0.094

0.511

0.082

Mercury (Hg)

0

0.128

All samples tested 0 for Mercury, just one sample tested 0.128 mg/l – probably due to contamination of this single sample.

Lead (Pb)

0

0.064

0.015


 

 TABLE 2A: MINERAL RESULTS COMPARED TO WHAT IS CURRENTLY PRINTED ON PACKAGING

 

Values below given in mg per 200 mlextract – in other words in a typical cup of tea / 1 portion

The values in italics are those where recent tests found similar levels as those currently used on packaging.

The values in bold – not in line with some current packaging, consider adjusting when reprinting packaging.

 

Mineral

Typical values currently printed on Rooibos packaging*

Values found during 2009 testing
(Tests done at Bemlab, 25 samples analysed, commissioned by SARC)

Lowest

Highest

Average*

Calcium (Ca)

1.09

0.46

2.46

1.06

Iron (Fe)

0.07

0.008

0.03

0.012

Copper (Cu)

0.07

0.002

0,006

0.004

Magnesium (Mg)

1.57

0.922

2.54

1.6

Manganese (Mn)

0.04

0.024

0.054

0.032

Potassium (K)

7.12

5.54

10.41

7.73

Sodium (Na)

6.16

5.54

10.85

7.60

Zinc (Zn)

0.04

0.004

0.012

0.008

Fluoride

0.22

<0.2 (below detection limit)

 

*These values used currently were probably sourced from tests done in the 1980s at the Institute for Fruit and Fruit Technology. Due to changes in soil conditions, these values need to be updated.

All these values are too low to make any health claims regarding the mineral content of Rooibos – see table and notes below.

 

TABLE 2B: RESULTS VS RDA VALUES

 

Mineral

Average mg/litre

Average mg in a
200 ml serving

RDA (mg) **

% RDA in one serving***

Calcium (Ca)

5.32

1.064

1 100

0.1

Iron (Fe)

0.06

0.012

14

0.09

Copper (Cu)

0.02

0.004

900*

0.0004

Magnesium (Mg)

8.00

1.6

350

0.46

Manganese (Mn)

0.16

0.032

2.3*

1.39

Potassium (K)

38.66

7.732

4 700*

0.16

Sodium (Na)

38.02

7.6

Must be less than
2 400 mg per day

Permissible claim: Very low in Sodium****

Zinc (Zn)

0.04

0.008

15 mg

0.05

Selenium

0.004

0.0008

0.055 mg

1.45

Fluoride

Below detection limit

-

10 mg

-

Vitamin A

Below detection limit

-

800 microgram

-

Vitamin C

Below detection limit

-

75 mg

-

Caffeine

None

None

-

-

 

*RDA values for Cu (Copper), Mn (Manganese), K (Potassium) not specified in this Government Gazette. These RDA values were obtained from a nutrition consultant.

 

**RDA values as published in Government Gazette of 8 August 2002, No 23714.

According to new legislation currently in the pipeline these RDA values will be changed to NRV values in future (NRV = Nutrient Reference Values) – but these values are expected to be very similar to current RDA values.

 

***To claim “source of” the foodstuff must provide 15% of the Minimum Dietary Requirement (MDR value) per serving and to claim “high in” the foodstuff must provide at least 30% of the MDR value per serving - Government Gazette No 30075 of 20 July 2007. All these values are therefore too low to make any health claim (with the exception of “very low in sodium” – see below).

 

(According to the new proposed food law foods containing >/= 5% NRV (no longer RDA, now called nutrient reference values) for a nutrient may be listed on pack. AND ONLY CLAIMS will be permitted if products have 15% NRV = source, 30% NRV = high in 60% = very high in.)

 

****Lower than 40mg per 100g can claim “very low in sodium” according to Government Gazette No 30075 of 20 July 2007

 

 

 

Marina Joubert

For: SA Rooibos Council

marina@southernscience.co.za